10.60933/PRDR/RWJUCXLee, Leo Man YuenLeo Man YuenLee0000-0002-2089-9311Hong Kong Polytechnic UniversityLeung, Thomas Yun-chungThomas Yun-chungLeung0000-0003-3590-7027Hong Kong Polytechnic UniversityPolyU Research Data Repository2024Medicine, Health and Life SciencesLee, Leo Man YuenLeo Man YuenLeeHong Kong Polytechnic University2024-05-302024-06-0610.3390/ijms24119402216074879821629651581998289600200195095523147358021924932821190730896526231632552485533710application/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-stream1.0CC BY-NC 4.0Organism: Mus musculus<br> Summary: It has been reported that low concentration of lysine suppressed lipid accumulation and transcription of several adipogenic genes in 3T3-L1 preadipocytes, however, the detailed lysine deprivation-specific cellular transcriptomic changes and the altered pathways have not yet been fully decrypted.<br> Overall design: Using 3T3-L1 cells, we performed RNA sequencing on undifferentiated cells, differentiated cells, and differentiated cells under a lysine-free culture medium